Transcription in the cyanobacterium Anabaena 7120 RNA polymerase structure and activity by George John Schneider

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Statementby George John Schneider.
Classifications
LC ClassificationsMicrofilm 88/272 (Q)
The Physical Object
FormatMicroform
Paginationviii, 122 leaves.
Number of Pages122
ID Numbers
Open LibraryOL2161334M
LC Control Number88890272

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Transcription of the Anabaena sp. strain PCC ntcA gene: multiple transcripts and NtcA binding. Department of Biology, Texas A&M University, College StationUSA. This article has been cited by other articles in by: While a few attempts to heterologously express cyanobacterial natural products have occurred, the results have been of varied success.

Here, we report the first steps in developing the model freshwater cyanobacterium Anabaena sp. Transcription in the cyanobacterium Anabaena 7120 book strain PCC (Anabaena ) as a general heterologous expression host for cyanobacterial secondary by: RNA polymerase from vegetative cells of the cyanobacterium Anabaena sp.

PCC comprises proteins with Ma of, 66, 52, named B'. Anabaena sp. strain PCCa filamentous cyanobacterium, is simultaneously capable of oxygenic photosynthesis and nitrogen fixation. Ni-trogen fixation is closely associated with cellular differentiation and intercellular interactions.

In the Transcription in the cyanobacterium Anabaena 7120 book of ammonium or nitrate, filaments of Anabaena sp. are comprised solely of pho-Cited by: (29) and nrrA (35) of Anabaena sp. strain PCC Previously (42), RNA polymerase (RNAP) was isolated from vegetative cells of the heterocyst-forming cyanobacterium Anabaena sp.

strain PCCand in vitro assays have shown this protein to initiate transcription at a number of vegetative cell promoters but not at a promoter of glnA that is Cited by: The transcription factor NtcA from the cyanobacterium Anabaena PCC was heterologously expressed in Escherichia coli.

In order to optimize the expression of NtcA, random silent mutations were introduced at the 5′ end of the DNA encoding the protein. To get as Cited by: Cyanobacterium Anabaena sp. Strain PCC PCC(referredtohereas Anabaena),aclusterof9genes(alltoall),ofwhich7had verse transcription-PCR (RT-PCR).

As shown in Fig. 3A, tran-scripts overlapping contiguous ORFs existed from all to. Transcriptional analysis of the rbcR1 and ntcA genes of Anabaena A: The rbcR1 Northern blot analysis of Anabaena cells grown under either ammonium (NH 4 +) supplemented or ammonium-free (NH 4 +-free) conditions.

The cells were treated for 2 h in either cold stress (20°C), sucrose stress ( mM) or salt stress (60 mM).Cited by: Anabaena PCC is a filamentous photosynthetic cyanobacterium that is composed of only vegetative cells when grown on combined nitrogen.

When nitrogen is limiting, however, about 1 in every 10 cells is transformed into a heterocyst [3].Cited by: 5. Gene transcription in filamentous cyanobacteria Article Literature Review (PDF Available) in Microbiology (Pt 3)(3) April with 25 Reads How we measure 'reads'.

Anabaena sp. PCC MATERIALS AND METHODS Bacterial strains. The wild-type Anabaena sp. strain PCC and its derivatives used in this study are listed in Table S1 in the supplemental material.

For the bioluminescent reporter strains, the bp 5= up-stream regions (5=-USRs) from the coding regions of pecB (alr) andCited by: (f) The psbA promoter The psbAI gene, whose promoter is cloned in pAn, is one member of a family encoding the D 1 protein of the photosystem II reaction center.

This promoter and the psbAl gene account for nearly all psbA transcription in Anabaena (Curtis and Haselkorn, ; Vrba and Curtis, ).Cited by: The filamentous, heterocystous cyanobacterium Nostoc sp. strain PCC (Anabaena sp. strain PCC ) possesses an uptake hydrogenase and a bidirectional enzyme, the latter being capable of catalyzing both H 2 production and evolution.

The completely sequenced genome of Nostoc sp. strain PCC reveals that the fiveCited by:   Abstract. Using differential display, we identified the Anabaena sp. PCC ribulose 1,5-bisphosphate carboxylase transcriptional regulator (rbcR1) gene, a member of the LysR family of positive transcription rbcR1 transcript and its putative target gene ribulose 1,5-bisphosphate carboxylase/oxygenase (rbcL/S) were repressed by cold (20°C) and osmotic (sucrose and salt) by:   Abstract.

When growing on N 2 as sole nitrogen source, the filamentous cyanobacterium Anabaena sp. PCC forms N 2 fixing heterocysts in a semi-regular pattern. To identify genes involved in heterocyst differentiation we characterised five transposon-generated mutants that were not able to form mature by:   Growth Conditions and Membrane Separation.

Axenic cultures of Anabaena PCC were grown photoautotrophically in BG medium at 35 °C for 1 week under illumination and bubbled with filtered air containing 1% (v/v) of CO were harvested by centrifugation ( × g, 10 min, 4 °C), washed twice with Hepes-EDTA buffer (10 m m Hepes/NaOH, pH5 m m NaCl, 2 m m Na 2.

Haselkorn R., Robinson S.J., Rice D. () Organization and Transcription of Nitrogen Fixation Genes in the Cyanobacterium Anabaena. In: Ciferri O., Dure L. Author: Robert Haselkorn, Steven J. Robinson, Douglas Rice. Golden JW, Whorff LL, Wiest DR. Independent regulation of nifHDK operon transcription and DNA rearrangement during heterocyst differentiation in the cyanobacterium Anabaena sp.

strain PCC J Bacteriol. Nov; (22)– [PMC free article]. The cyanobacterium Anabaena (Nostoc) sp. strain PCC grows as long filaments of photosynthetic vegetative cells in the presence of combined nitrogen. In an environment lacking combined nitrogen, about 7 to 10% of the cells terminally differentiate into nitrogen-fixing by: A vector for analysis of promoters in the cyanobacterium Anabaena sp.

strain PCC Article (PDF Available) in Journal of Bacteriology (8) May with 18 Reads How we measure 'reads'. Expression in the Cyanobacterium Anabaena sp. Strain PCC DANUTA CHAMOT AND GEORGE W. OWTTRIM* Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 Received 2 September /Accepted 5 December Expression of the Anabaena sp.

strain PCC RNA helicase gene crhC is induced by cold shock. crhCCited by:   In the filamentous, heterocystous cyanobacterium Anabaena sp.

PCCisiA and the gene for flavodoxin are located in separate operons with independent promoters. In this paper, we report on the sequence of isiA and show that it is found in a monocistronic operon that is transriptionally regulated to be expressed under iron stress but does Cited by:   In the nitrogen-fixing cyanobacterium Anabaena sp.

strain PCC the presence of three open reading frames (all, all and alr) putatively encoding proteins with the histidine-rich region characteristic of the Fur protein family has been identified by genome sequence by: The hetR, patA, hetN, and patS genes are part of a regulatory network that regulates the differentiation and patterning of heterocysts in the filamentous cyanobacterium Anabaena sp.

strain PCC In this report, the epistatic interactions of mutant alleles of these four genes have been used to refine our understanding of their relationships to one by: The structural gene for ferredoxin I, petF, from the cyanobacterium Anabaena sp.

strain PCC has been isolated from a recombinant lambda by: Anabaena sp. PCC (in the following referred to as Anabaena sp.) has been identified by proteomic analysis (18, 19) and its relation to the TolC family was confirmed by homology modeling and analysis of the corresponding mutant (5, 7, 20).

Anabaena sp. is a filamentous, photosynthetic cyanobacterium. This thesis concerns different molecular aspects of the transcription factor NtcA from the heterocystous cyanobacterium Anabaena PCC Apart from performing oxygenic photosynthesis, Anabaena PCC is also capable of fixing nitrogen. NtcA is a protein regulating transcription of a wide range of genes and in particular genes involved in.

Abstract. Glutathione reductase (GR) was purified from the cyanobacterium Anabaena PCC A 3-kilobase genomic DNA fragment containing the coding sequence for the GR gene (gor) was identified and cloned by polymerase chain reaction based on sequences of selected peptides isolated from proteolyzed coding sequence encompassing amino acid residues, as well Cited by: Among the 4 predicted ftsH genes in the cyanobacterium Anabaena sp.

PCCnamely alr, all, all and all, at least the first two showed reduced transcription in specialized N2-fixing cells called heterocysts. In contrast, the transcription of a small internal antisense RNA (aftsH) of all was up-regulated in by: 1.

The filamentous cyanobacterium Anabaena sp. strain PCC forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen.

PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start. The filamentous cyanobacterium Anabaena sp. strain PCC differentiates specialized cells for nitrogen fixation called heterocysts upon limitation of combined nitrogen in the medium.

During heterocyst differentiation, expression of approximately genes is upregulated with spatiotemporal by: 8. Anabaena sp. PCC (A. ) is a filamentous cyanobacterium. Upon depletion of combined nitrogen in the medium, with a regular interval of 10–15 cells, vegetative cells differentiate into heterocysts, which are terminally differentiated cells specialized for nitrogen fixation (Kumar et al.

).Cited by: 6. Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena William J. Buikema and Robert Haselkorn Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois USA Anabaena mutant fails to differentiate heterocysts.

An amidase is required for proper intercellular communication in the filamentous cyanobacterium Anabaena sp. PCC Zhenggao Zhenga, Amin Omairi-Nasserb, Xiying Li a, Chunxia Dong, Yan Linc, Robert Haselkornb,1, and Jindong Zhaoa,c,1 aState Key Laboratory of Protein and Plant Genetic Engineering, College of Life Sciences, Peking University, BeijingPeople’s Republic of China;Cited by: The FurA regulon in Anabaena sp.

PCC in silico prediction and experimental validation of novel target genes Andre´s Gonza´lez1,2,*, Vladimir Espinosa Angarica1,2,3, Javier Sancho1,2,3 and. filamentous cyanobacterium Anabaena sp. strain PCC (hereafter referred to as Anabaena PCC ), about genes are upregulated with spatiotemporal regulation during the process of heterocyst development [3–6].

Two genes, ntcA and hetR, are necessary for heterocyst differentiation,Cited by: 8. Cyanobacteria Flavodiiron protein Heterocyst Oxidative stress Anabaena sp. PCC abstract Flavodiiron proteins present in many prokaryotic and some eukaryotic organisms have a capacity to protect cells against nitrosative or oxidative stress.

In Anabaena sp. PCCFlv1 and Flv3 proteins are encoded by families of two by:   Abstract. Anabaena sp. PCC is a filamentous cyanobacterium that bears a cluster of 26 tRNA genes and pseudogenes in the delta plasmid. The sequences of these tRNAs suggest that they have been acquired by horizontal gene transfer from another organism.

The cluster is transcribed as a single transcript that is quickly processed to individual by: 9. Structure of transcription factor HetR required for heterocyst differentiation in cyanobacteria Youngchang Kima,1, Grazyna Joachimiaka,1,ZiYeb,c, T.

Andrew Binkowskia, Rongguang Zhanga, Piotr Gornickib, Sean M. Callahand, Wolfgang R. Hesse, Robert Haselkornb,2, and Andrzej Joachimiaka,f,2 aMidwest Center for Structural Genomics and Structural Biology Center, Biosciences, Argonne Cited by: The transcription factor NtcA from the cyanobacterium Anabaena PCC was heterologously expressed in Escherichia coli.

In order to optimize the expression of NtcA, random silent mutations were introduced at the 5′ end of the DNA encoding the protein. To get as high a yield of pure protein as possible, different strategies of expression as well as purification conditions were used.

Under. The early decline in the expression of the ntcA, rbcR1, and rbcL/S transcripts appears to be part of the Anabaena global adaptation response to stress. The substantial increase in the ntcA gene expression 24 h following cold stress suggests that Anabaena experiences substantial nitrogen limitation under these by: Anabaena sp.

PCC is a filamentous cyanobacterium. In the presence of fixed nitrogen, usua11y nitrate or ammonium, all the cells in the filament are vegetative cells. In the absence of fixed nitrogen, vegetative cells differentiate into heterocysts at semiregular intervals.

Thus.Using differential display, we identified the Anabaena sp. PCC ribulose 1,5‐bisphosphate carboxylase transcriptional regulator (rbcR1) gene, a member of the LysR family of positive transcription rbcR1 transcript and its putative target gene ribulose 1,5‐bisphosphate carboxylase/oxygenase (rbcL/S) were repressed by cold (20°C) and osmotic (sucrose and salt) by:

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